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Cancer Functional Genomics

Gene expression variability across the genome has significant impact on understanding the progress and prognosis of cancer. The Cancer Functional Genomics Shared Resource at the Stephenson Cancer Center offers cutting-edge technology that can provide extremely accurate and reliable expression data to support drug discovery research. The versatile Agilent SureScan Microarray Scanner system provides the ability to scan genome-wide microarray profiles.

For quality assessment of purified RNA and DNA, there are two instruments that include the Biorad Experion™ Automated Electrophoresis Station and Agilent 2100 Bioanalyzer. The Biorad CFX96™ Touch Real-Time PCR Detection System provides highly-reliable quantitative individual gene transcription profiling. Bio-Rad QX100 Droplet Digital PCR is useful to detect the absolute copy number of the genes.

Functional analysis of proteins using biochemical assay can be evaluated with the Perkin Elmer EnVision® Multilabel Reader. An ultimate high-throughput high-content imaging instrument, Operetta from Perkin Elmer can provide high resolution images for screening drugs under live and fixed cell context. Live cell metabolic changes with respect to oxygen consumption and pH change due to respiration can be determined using Xfe 96 extracellular flux analyzer exclusively from Seahorse.

Services

  • Array Scanning and Quantification
  • Reverse Proteomics
  • Array Real-Time PCR
  • Multimodal Assay Screening
  • DNA / RNA / Protein Purity Analysis on a Chip
  • High-content Drug Screening
  • Metabolic Analysis of Live Cells
  • Absolute Allele Copy Number Determination
  • Clonogenic colony and spheroid counting

Equipment

  • Agilent SureScan Microarray Scanner System
  • Arrayit Spotware Colormetric Microarray Scanner
  • Biorad CFX96™ Touch Real-Time PCR Detection System
  • Biorad Experion™ Automated Electrophoresis Station and Agilent 2100 Bioanalyzer
  • Biorad QX100 Droplet Digital PCR
  • Janus Automated Workstation
  • Molecular Devices GenePix 4100A Microarray Scanner
  • Nanoprint Microarray Printing
  • Oxford Optronix GelCountTM Colony Counter
  • Perkin Elmer EnVision® Multilabel Reader
  • Perkin Elmer Operetta
  • XFe 96 Extracellular Flux Analyzer

Contact Information

Muralidharan Jayaraman, PhD
Director of Research Core Operations
405-271-6890
muralidharan-jayaraman@ouhsc.edu

Core Hours

Monday through Friday
8 am to 5 pm